The Needle in the DNA-stack

No Need to Look for the Needle in the DNA-stack When You Can Create One Yourself. Many professionals in plant business are familiar with the recent introduction of a technology, called “genome editing”, which has the potential to change breeding dramatically. The most commonly used genome editor is the Crispr/CAS system. Crispr/CAS can make a mutation at specific positions in any plants’ DNA, which may result in a better trait. In nature, mutations are induced all the time. Over thousands of years this is what has created the variation upon which we build all new varieties today. But not all mutations are easily detected.

The problem is that most mutations with a positive effect are ‘silent’ because the plant contains another gene copy, which renders the altered function of the mutated gene invisible. The only way to discover the mutation is then to sequence the gene(s) behind the trait in thousands, if not millions, of individuals. In the past and even today researchers accelerated the induction of mutations in plants by treating seeds either with chemicals or radiation. While this indeed created many more mutations in each plant, not all of them are beneficial, and years of backcrossing into elite material is normally needed to remove all unwanted mutations.

This is why plant researchers and breeders find genome editing so appealing:

• It is clean – induces one mutation and that’s it
• it saves time – no tedious searching through individuals or sequences, no prolonged backcrossing
• it is easy to track through the breeding process because the mutation and its effect is easily detected

Does the system work in grasses? Yes, it does, and currently DLF and other breeding companies and universities are exploring the options to utilize Crispr/CAS to improve yield and quality in grasses, alfalfa, cereals, and potato. The activities are part of a major Danish project called ReTraQue, which received financial support from the Innovation Fund Denmark. Improved yield can be obtained by mutating genes in the photosynthetic system and improved forage quality can be obtained by reducing the effect of genes responsible for lignin production in stems and leaves.

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